Gel Crusher Spin Column, PP, 600µm Pore

• Gel Crusher Spin Column / Strainer Basket, ten 600µm pores, PP, for gel fragmentation and gel breaking processes, fits 2 mL receiver tubes, 200/pk • MTC Bio's Gel Crusher Spin Columns are designed to replace labor-intensive gel fragmentation steps (crush and soak method, etc.). • Commonly used in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols, these spin columns can be used to fragment gel slices (typically cut with a gel extractor or razor blade) into smaller pieces. • These smaller uniform pieces will produce greater yields and can be easily pipetted using wide bore tips into an NaCl solution. • This reduces the time to create a fine slurry for further elution, filtration, incubation, etc. Place the Gel Crusher column in the 2 mL receiver tube and centrifuge the column/tube assembly in a standard benchtop microcentrifuge for 5 minutes at maximum speed (approximately 15,000×g).The gel will be forced through the 600µm pores into the 2 mL receiver tube. • The crushed fragments will be small enough to be aspirated/dispensed through a pipette tip. • Creates uniform gel fragments with no chunks • Fits in standard microtubes • Increases DNA yield • For use in molecular cloning, PCR, restriction digestions, sequencing and labeling protocols.